FOM Institute AMOLF 1 article published in JoVE Bioengineering Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration Nicholas Agung Kurniawan1, Parthiv Kant Chaudhuri2, Chwee Teck Lim2,3 1Systems Biophysics Department, FOM Institute AMOLF, 2Mechanobiology Institute, National University of Singapore, 3Department of Biomedical Engineering, National University of Singapore The mechanical properties and microstructure of the extracellular matrix strongly affect 3D migration of cells. An in vitro method to study the spatiotemporal cell migration behavior in biophysically variable environments, at both population and individual cell levels, is described.