University of Florida College of Medicine 1 article published in JoVE Developmental Biology A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle Connor D. Johnson1, Lylybell Y. Zhou1, Daniel Kopinke1,2 1Department of Pharmacology and Therapeutics, University of Florida College of Medicine, 2Myology Institute, University of Florida College of Medicine The replacement of healthy muscle tissue with intramuscular fat is a prominent feature of human diseases and conditions. This protocol outlines how to visualize, image, and quantify intramuscular fat, allowing the rigorous study of the mechanisms underlying intramuscular fat formation.