The University of North Carolina at Chapel Hill School of Medicine 1 article published in JoVE Biology Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions Meredith G. Chambers1, Ryan P. McNamara1, Dirk P. Dittmer1 1Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill School of Medicine Direct stochastic optical reconstruction microscopy (dSTORM) is used to bypass the typical diffraction limit of light microscopy and to view exosomes at the nanometer scale. It can be employed in both two and three dimensions to characterize exosomes.