North Dakota State University 4 articles published in JoVE Bioengineering Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds Bharat Kumar Reddy Sanapalli1, Kalaivani Chinna Gounder1, Nilesh Sudhakar Ambhore2, Gowthamarajan Kuppuswamy1, Praveen Thaggikuppe Krishnamurthy3, Veera Venkata Satyanarayana Reddy Karri1 1Department of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education & Research, 2Department of Pharmaceutical Sciences, School of Pharmacy, North Dakota state university, 3Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education & Research The prepared DOX-CL scaffold satisfied the prerequisites of an ideal DW dressing in mechanical strength, porosity, water absorption, degradation rate, sustained release, anti-bacterial, biocompatibility, and anti-inflammatory properties, which are considered to be essential for the recovery of damaged tissue in DWs. Bioengineering Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes Meihua Wan1,2, Pouya Amrollahi2,3, Dali Sun4, Christopher Lyon2,3, Tony Y. Hu2,3 1Department of Integrated Traditional Chinese and Western Medicine, West China Hospital of Sichuan University, 2Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute, Arizona State University, 3School of Biological and Health Systems Engineering, Arizona State University, 4Department of Electrical and Computer Engineering, North Dakota State University Clinical translation of exosome-derived biomarkers for diseased and malignant cells is hindered by the lack of rapid and accurate quantification methods. This report describes the use of low-magnification dark-field microscope images to quantify specific exosome subtypes in small volume serum or plasma samples. Environment A Precise and Autonomous System for the Detection of Insect Emergence Patterns Meghan M. Bennett1,2, Joseph P. Rinehart3, George D. Yocum3, Ian Yocum3 1Department of Biological Sciences, North Dakota State University, 2School of Life Sciences, Arizona State University, 3Red River Valley Agricultural Research Center, USDA-ARS Measurement of insect emergence patterns requires precision. Existing systems are only semi-automated and sample size is limited. We addressed these issues by designing a system using microcontrollers to precisely measure the time of emergence of large numbers of emerging insects. Biology Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm Aurelia C. Kucera1, Britt J. Heidinger2 1Environmental and Conservation Sciences Program, North Dakota State University, 2Department of Biological Sciences, North Dakota State University This protocol describes cloacal massage, a non-invasive manual technique for collecting semen from birds, and DNA extraction from avian sperm using a commercial extraction kit with modifications.