West Virginia University School of Medicine 4 articles published in JoVE Immunology and Infection A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis Brittany G. Seman*1, Jessica M. Povroznik*1,2, Jordan K. Vance1, Travis W. Rawson1, Cory M. Robinson1,2 1Department of Microbiology, Immunology, & Cell Biology, West Virginia University School of Medicine, 2Vaccine Development Center at West Virginia University Health Sciences Center Infection of neonatal mice with bioluminescent E. coli O1:K1:H7 results in a septic infection with significant pulmonary inflammation and lung pathology. Here, we describe procedures to model and further study neonatal sepsis using longitudinal intravital imaging in parallel with enumeration of systemic bacterial burdens, inflammatory profiling, and lung histopathology. Cancer Research In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment Andrey A. Bobko1,2, Timothy D. Eubank1,3, Benoit Driesschaert1,2, Valery V. Khramtsov1,2 1In Vivo Multifunctional Magnetic Resonance center, Robert C. Byrd Health Sciences Center, West Virginia University, 2Department of Biochemistry, West Virginia University School of Medicine, 3Department of Microbiology, Immunology & Cell Biology, West Virginia University School of Medicine Low-field (L-band, 1.2 GHz) electron paramagnetic resonance using soluble nitroxyl and trityl probes is demonstrated for assessment of physiologically important parameters in the tumor microenvironment in mouse models of breast cancer. Medicine Modeling Chemotherapy Resistant Leukemia In Vitro William L. Slone*1, Blake S. Moses*1, Rebecca Evans1, Debbie Piktel1, Karen H. Martin1,2, William Petros1, Michael Craig1, Laura F. Gibson1,3 1Alexander B. Osborn Hematopoietic Malignancy and Transplantation Program of the Mary Babb Randolph Cancer Center, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine, 2Department of Neurobiology and Anatomy, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine, 3Department of Microbiology, Immunology and Cell Biology, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine The current report summarizes a protocol that can be utilized to model the influence of the bone marrow microenvironment niche on leukemic cells with emphasis placed on enrichment of the most chemoresistant subpopulation. Behavior Asymmetric Walkway: A Novel Behavioral Assay for Studying Asymmetric Locomotion Kiril Tuntevski1, Ryan Ellison1, Sergiy Yakovenko1 1Neural Engineering Laboratory, Biomedical Research Center, West Virginia University School of Medicine Here, we present a protocol to quantify precise stepping in rodents. Cortical and the spinal central pattern generator signals are required for precise foot-placement during obstructed locomotion. We report here the novel constrained walking task that directly examines precise stepping behavior.