Merrimack College View Institution's Website 1 article published in JoVE Immunology and Infection Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells Charlotte Berkes1,2, Leo Li-Ying Chan2,3, Alisha Wilkinson1,2,3, Benjamin Paradis2,3 1Department of Biology, Merrimack College, 2Center for Biotechnology and Biomedical Sciences, Merrimack College, 3Department of Technology R&D, Nexcelom Bioscience LLC Here, we demonstrate how image cytometry can be used for quantification of pathogenic fungi in association with host cells in culture. This technique can be used as an alternative to CFU enumeration.