Albany Medical College View Institution's Website 7 articles published in JoVE Cancer Research Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment Cassandra L. Roberge1, Ling Wang2, Margarida Barroso2, David T. Corr1 1Rensselaer Polytechnic Institute, 2Albany Medical College The present protocol develops an image-based technique for rapid, non-destructive, and label-free regional cell density and viability measurement within 3D tumor aggregates. Findings revealed a cell-density gradient, with higher cell densities in core regions than outer layers in developing aggregates and predominantly peripheral cell death in HER2+ aggregates treated with Trastuzumab. Cancer Research Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter Yuxuan Xiao1, John M. Lamar1 1Department of Molecular & Cellular Physiology, Albany Medical College To identify novel regulators of transcription factors, we developed an approach to screen arrayed lentiviral or retroviral RNAi libraries using a dual-luciferase-based transcriptional reporter assay. This approach offers a quick and relatively inexpensive way to screen hundreds of candidates in a single experiment. Cancer Research Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs Janine S. A. Warren1, Paul J. Feustel2, John M. Lamar1 1Department of Molecular and Cellular Physiology, Albany Medical College, 2Department of Neuroscience and Experimental Therapeutics, Albany Medical College The described approach combines experimental tail vein metastasis assays with in vivo live animal imaging to allow real-time monitoring of breast cancer metastasis formation and growth in addition to the quantification of metastasis number and size in the lungs. Immunology and Infection Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin Ramkumar Mathur1,2,3, Mahabub Maraj Alam4, Xiao-Feng Zhao4, Yunfei Huang4, Xinjun Zhu1,2 1Department of Molecular and Cellular Physiology, Albany Medical College, 2The IBD Center, Division of Gastroenterology, Department of Medicine, Albany Medical College, 3Department of Geriatrics, School of Medicine and Health Science, University of North Dakota, 4Department of Neuroscience and Experimental Therapeutics, Albany Medical College In this study, we describe a detailed procedure of TNBS-mediated intestinal fibrosis, which exhibits comparable pathophysiology to Crohn's fibrosis. We also discuss this approach in light of rapamycin facilitated inhibitory effects on intestinal fibrosis. Biology Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans Shaarika Sarasija1, Kenneth R. Norman1 1Department of Regenerative and Cancer Cell Biology, Albany Medical College Mitochondrial respiration is critical for organismal survival; therefore, oxygen consumption rate is an excellent indicator of mitochondrial health. In this protocol, we describe the use of a commercially available respirometer to measure basal and maximal oxygen consumption rates in live, intact, and freely-motile Caenorhabditis elegans. Developmental Biology Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution Wasay M. Shaikh Qureshi1, Lianjie Miao1, David Shieh1, Jingjing Li1, Yangyang Lu1, Saiyang Hu1, Margarida Barroso1, Joseph Mazurkiewicz2, Mingfu Wu1 1Department of Molecular and Cellular Physiology, Albany Medical College, 2Department of Neuroscience and Experimental Therapeutics, Albany Medical College We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately. Neuroscience Recording Human Electrocorticographic (ECoG) Signals for Neuroscientific Research and Real-time Functional Cortical Mapping N. Jeremy Hill1, Disha Gupta1,2, Peter Brunner1,2, Aysegul Gunduz1,2, Matthew A. Adamo3, Anthony Ritaccio2, Gerwin Schalk1,2,4,5,6,7 1Wadsworth Center, New York State Department of Health, 2Department of Neurology, Albany Medical College, 3Department of Neurosurgery, Albany Medical College, 4Department of Neurosurgery, Washington University, 5Department of Biomed. Eng., Rensselaer Polytechnic Institute, 6Department of Biomed. Sci., State University of New York at Albany, 7Department of Elec. and Comp. Eng., University of Texas at El Paso We present a method for collecting electrocorticographic signals for research purposes from humans who are undergoing invasive epilepsy monitoring. We show how to use the BCI2000 software platform for data collection, signal processing and stimulus presentation. Specifically, we demonstrate SIGFRIED, a BCI2000-based tool for real-time functional brain mapping.