JOVE JOURNAL

생화학

Showing 193 - 204 in 724 videos.

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Frederik Steiert², Tamara Heermann, Nikolas Hundt, Petra Schwille

¹Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry, ²Department of Physics,Technical University Munich, ³Department of Cellular Physiology, Biomedical Center (BMC),Ludwig-Maximilians-Universität München

Journal (Biochemistry)

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Nandini Mani*^1,2, Michelle F. Marchan*¹, Radhika Subramanian²

¹Molecular Biology,Massachusetts General Hospital, ²Department of Genetics,Harvard Medical School

Journal (Biochemistry)

Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

Andreas Andric, Eva Wagner, Anne Heberle, Max Holzknecht, Alexander K. H. Weiss

¹Research Institute for Biomedical Aging Research,University of Innsbruck

Journal (Biochemistry)

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Lukas Pekarek, Stefan Buck, Neva Caliskan²

¹Helmholtz Institute for RNA-based Infection Research (HIRI),Helmholtz Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research), ²Medical Faculty,Julius-Maximilians University Würzburg

Journal (Biochemistry)

CD Spectroscopy to Study DNA-Protein Interactions

Vijendra Arya, Anindita Dutta, Rohini Muthuswami

¹Jawaharlal Nehru University New Delhi

Journal (Biochemistry)

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Robert P. Sparks*¹, William Lawless*², Andres S. Arango*^1,3,4, Emad Tajkhorshid^3,4, Rutilio A. Fratti³

¹Department of Biochemistry,University of Illinois at Urbana-Champaign, ²Department of Chemistry,University of South Florida, ³Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign, ⁴Beckman Institute for Advanced Science and Technology,University of Illinois at Urbana-Champaign

Journal (Biochemistry)

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

Ziyi Wang², Qinghua Liu⁴, Liang Wang⁵, Robert G. Gilbert^2,6, Mitchell A. Sullivan

¹School of Chemistry and Molecular Biosciences,The University of Queensland, ²Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation,The University of Queensland, ³Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy,Xuzhou Medical University, ⁴Department of Pharmaceutical Analysis, School of Pharmacy,Xuzhou Medical University, ⁵Department of Bioinformatics, School of Medical Informatics and Engineering,Xuzhou Medical University, ⁶Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture,Yangzhou University, ⁷Glycation and Diabetes Group,Mater Research Institute-The University of Queensland, Translational Research Institute

Journal (Biochemistry)

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Rasmus Jensen, Niels Ørtenblad, Cristiano di Benedetto, Klaus Qvortrup, Joachim Nielsen

¹Research center for applied health science,University College South Denmark, ²Department of Sports Science and Clinical Biomechanics,University of Southern Denmark, ³Department of Biomedical Sciences, Core Facility for Integrated Microscopy,University of Copenhagen

Journal (Biochemistry)

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Hoang P. M. Nguyen*¹, Kelly L. McGuire*¹, Brian D. Cook*¹, Mark A. Herzik, Jr.

¹Department of Chemistry and Biochemistry,University of California, San Diego

Journal (Biochemistry)

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

Himanshu Pandey, Tatiana Zvagelsky, Mary Popov, Mayan Sadan, Neta Yanir, Alina Goldstein-Levitin, Nurit Siegler, Shira Hershfinkel, Yahel Abraham, Roy Avraham, Levi A. Gheber, Larisa Gheber

¹Department of Chemistry,Ben-Gurion University of the Negev, Israel, ²Department of Biotechnology Engineering,Ben-Gurion University of the Negev, Israel

Journal (Biochemistry)

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Megan C. DiIorio, Arkadiusz W. Kulczyk²

¹Institute for Quantitative Biomedicine,Rutgers University, ²Department of Biochemistry & Microbiology,Rutgers University

Journal (Biochemistry)

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Jordan Demone, Kiah A. Barton², Illimar Altosaar²

¹Department of Biochemistry, Microbiology, and Immunology,University of Ottawa, ²Proteins Easy Corp

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