JOVE JOURNAL

생화학

Showing 445 - 456 in 724 videos.

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Ingo Bauer, Angelo Pidroni, Özgür Bayram³, Gerald Brosch, Stefan Graessle

¹Division of Molecular Biology, Biocenter,Medical University of Innsbruck, ²Biology Department,Maynooth University, ³Maynooth University Human Health Research Institute

Journal (Biochemistry)

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Rui Yin*, Catherine Harvey*, Diane C. Shakes*, Oliver Kerscher*

¹Integrated Science Center, Biology Department,College of William & Mary

Journal (Biochemistry)

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Alex Y. H. Wong, Sheng Xie, Ben Zhong Tang, Sijie Chen

¹Ming Wai Lau Centre for Reparative Medicine,Karolinska Institutet, ²Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science,Hong Kong University of Science and Technology

Journal (Biochemistry)

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Simon Leclerc², Youri Arntz, Yuichi Taniguchi³

¹Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research,RIKEN, ²Laboratoire de Biomatériaux et Bioimagerie,INSERM, ³PRESTO, Japan Science and Technology Agency

Journal (Biochemistry)

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Fatima Enam, Thomas J. Mansell

¹Department of Chemical and Biological Engineering,Iowa State University

Journal (Biochemistry)

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Yu-Ting Huang², Dinja van der Hoorn*^1,2, Leire M. Ledahawsky*^1,2, Anna A. L. Motyl*^1,2, Crispin Y. Jordan, Thomas H. Gillingwater², Ewout J. N. Groen²

¹Centre for Discovery Brain Sciences,University of Edinburgh, ²Euan MacDonald Centre for Motor Neurone Disease Research,University of Edinburgh

Journal (Biochemistry)

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization

Hadar Amartely, Daniel Some, Ayala Tsadok, Mario Lebendiker

¹Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science,The Hebrew University of Jerusalem, ²Wyatt Technology Corporation, ³Danyel Biotech Ltd

Journal (Biochemistry)

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

Casey S. O’Neil, Jacie L. Beach, Todd D. Gruber

¹Department of Molecular Biology and Chemistry,Christopher Newport University

Journal (Biochemistry)

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Stephanie Hutin, Bart Van Laer, Christoph Mueller-Dieckmann, Gordon Leonard, Didier Nurizzo, Matthew W. Bowler

¹Structural Biology Group,European Synchrotron Radiation Facility, ²Grenoble Outstation,European Molecular Biology Laboratory

Journal (Biochemistry)

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Stephanie Hutin*¹, Gianluca Santoni*¹, Ulrich Zander, Nicolas Foos, Sylvain Aumonier, Guillaume Gotthard, Antoine Royant³, Christoph Mueller-Dieckmann, Gordon Leonard

¹European Synchrotron Radiation Facility,Structural Biology Group, ²European Molecular Biology Laboratory, ³Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)

Journal (Biochemistry)

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Melaku Garsamo³, Yun Zhou³, Xing Liu³

¹Department of Biochemistry,Purdue University, ²Department of Botany and Plant Pathology,Purdue University, ³Center for Plant Biology,Purdue University

Journal (Biochemistry)

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Sebastian Flores, Andrew P. Feld, Bryan H. Thurtle-Schmidt

¹Department of Biology,Davidson College

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