All Protocols and Video Articles for scientific research | JoVE

Showing 37 - 48 of 164 videos for "INDEL"

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Journal (Genetics)

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

Rebeca Carballar-Lejarazú, Thai Binh Pham, Adam Kelsey, Taylor Tushar, Anthony A. James²

¹Department of Microbiology & Molecular Genetics,University of California, Irvine, ²Department of Molecular Biology & Biochemistry,University of California, Irvine

Journal (Neuroscience)

Acquisition of Resting-State Functional Magnetic Resonance Imaging Data in the Rat

Diana J. Wallin*^1,2, Emily D. K. Sullivan*^1,2, Elise M. Bragg, Jibran Y. Khokhar⁴, Hanbing Lu³, Wilder T. Doucette²

¹Dartmouth-Hitchcock Medical Center, ²Geisel School of Medicine at Dartmouth, ³National Institute on Drug Abuse,National Institutes of Health, ⁴University of Guelph

Journal (Genetics)

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연구 방법 컬렉션

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Changkun Hu*¹, Tyler Doerksen*², Taylor Bugbee, Nicholas A. Wallace, Rachel Palinski³

¹Division of Biology,Kansas State University, ²Kansas State Veterinary Diagnostic Laboratory,Kansas State University, ³Department of Diagnostic Medicine/Pathobiology,Kansas State University

Journal (Biochemistry)

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연구 방법 컬렉션

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Claudine Bisson², Corey W. Hecksel⁴, James B. Gilchrist, M. Alejandra Carbajal, Roland A. Fleck

¹Centre for Ultrastructural Imaging,New Hunt’s House, Guy’s Campus, King’s College London, ²Department of Biological Science,Birkbeck College, University of London, ³Electron Bio-Imaging Centre,Diamond Light Source, Harwell Science and Innovation Campus, ⁴SLAC National Accelerator Laboratory,Stanford University

Journal (Chemistry)

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연구 방법 컬렉션

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

Letizia Barbieri², Enrico Luchinat³

¹Magnetic Resonance Center − CERM,University of Florence, ²Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine − CIRMMP, ³Neurofarba Department,University of Florence

Journal (Immunology and Infection)

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연구 방법 컬렉션

Production of Human CRISPR-Engineered CAR-T Cells

Sangya Agarwal*^1,2, Nils Wellhausen*^1,2, Bruce L. Levine², Carl H. June²

¹Center for Cellular Immunotherapies, Perelman School of Medicine,University of Pennsylvania, ²Parker Institute for Cancer Immunotherapy,University of Pennsylvania

Journal (Neuroscience)

Analysis of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage with High Frequency Transcranial Duplex Ultrasound

Axel Neulen*¹, Michael Molitor*^2,3,4, Michael Kosterhon*¹, Tobias Pantel, Susanne H. Karbach^3,4, Philip Wenzel^3,4, Thomas Gaul, Florian Ringel, Serge C. Thal

¹Department of Neurosurgery,University Medical Center of the Johannes Gutenberg-University of Mainz, ²Center for Cardiology - Cardiology I,University Medical Center of the Johannes Gutenberg-University of Mainz, ³Center for Thrombosis and Hemostasis (CTH),University Medical Center of the Johannes Gutenberg-University of Mainz, ⁴German Center for Cardiovascular Research (DZHK) - Partner site Rhine-Main, ⁵Department of Anesthesiology,University Medical Center of the Johannes Gutenberg-University of Mainz

Journal (Genetics)

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연구 방법 컬렉션

Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Isabelle Louradour, Kashinath Ghosh, Ehud Inbar, David L. Sacks, Channa Aluvihare, Robert A. Harrell II

¹Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health, ²University of Maryland Insect Transformation Facility,The Institute for Bioscience and Biotechnology Research

Journal (Developmental Biology)

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Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica

Takahiro Ohde, Toshinori Minemura, Eiichi Hirose, Takaaki Daimon

¹Department of Applied Biosciences, Graduate School of Agriculture,Kyoto University

Journal (Developmental Biology)

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Kara A. Nelson, Bailey N. Warder, Stephen DiNardo, Lauren Anllo

¹Cell and Developmental Biology Department and the Penn Institute for Regenerative Medicine, Perelman School of Medicine,University of Pennsylvania

Journal (Biology)

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연구 방법 컬렉션

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Kanut Laoharawee^2,3, Matthew J. Johnson^2,3, Walker S. Lahr^2,3, Joseph J. Peterson^2,3, Beau R. Webber^2,3, Branden S. Moriarity^2,3

¹Department of Pediatrics,University of Minnesota, ²Center for Genomic Engineering,University of Minnesota, ³Masonic Cancer Center,University of Minnesota

Journal (Biology)

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연구 방법 컬렉션

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

Megan E. Meuti, Robert Harrell

¹Department of Entomology,The Ohio State University, ²Insect Transformation Facility, Institute for Bioscience and Biotechnology Research,University of Maryland

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