Airway physiology measurement software (Rescomp) was custom prepared (Millenium Premier Group; 415-519-4371). Data was analyzed using a PC workstation running Windows XP equipped with a Pentium III CPU (Intel, Inc. Santa Clara, CA) and a 17-pin analog to digital signal converter (National Instruments, #PC-LPM16). A small animal airway physiology workstation was custom assembled (Millenium Premier Group) using commercially available pressure transducers (part #TRD5700 and TRD4510), preamp modules (part #MAX2270), chassis (part # MAX1320; all from Buxco, Inc. Wilmington, NC) and a customized small animal plethysmograph. 0.5mm external diameter fiber-optic thread, connected to light source (Cole Palmer Illuminator, 41722 series) Ventilator (Harvard Apparatus Mouse Ventilator, #687) 10 mm, 27ga needle (BD Biosciences, cat. no. 309602) Heat lamp 1 ml syringe (BD Biosciences, cat. no. 305109) 4 clamps (Pony 3200 spring clamp) 0.5 mm external wire for intubation guide Hemacytometer Superfrost/plus microscope slides (Fisher cat. no. 12-550-15) Shandon Filter Cards (Thermo cat. no. 5991022) Differential cell slide stain (Fisher cat. no. 122911) Light microscope (Leica) Cytospin 3 (Shandon) 20 ga, 1.25 inch ProtectIV intravenous catheters (Smith Medical) 0.5 mm polymer optical fiber (Edmund Optics # NT02-532).
<!– OLD Materials List 4/12/10 Changed
Airway physiology measurement software (Rescomp) was custom prepared (Millenium Premier Group; 415-519-4371) and data were analyzed using a PC workstation running Windows XP equipped with a Pentium III CPU (Intel, Inc. Santa Clara, CA) and a 17-pin analog to digital signal converter (National Instruments, #PC-LPM16). Small animal airway physiology workstation was custom assembled (Millenium Premier Group) using commercially available pressure transducers (part #TRD5700 and TRD4510), preamp modules (part #MAX2270) and chassis (part # MAX1320; all from Buxco, Inc. Wilmington, NC) and a customized small animal plethysmograph. 0.5mm external diameter fiber-optic thread, connected to light source (Cole Palmer Illuminator, 41722 series); Ventilator (Harvard Apparatus Mouse Ventilator, #687); 10 mm, 27ga needle (BD Biosciences, cat. no. 309602); Heat lamp; 1 ml syringe (BD Biosciences, cat. no. 305109); 4 clamps (Pony 3200 spring clamp); 0.5 mm external wire for intubation guide); Hemacytometer; Superfrost/plus microscope slides (Fisher cat. no. 12-550-15); Shandon Filter Cards (Thermo cat. no. 5991022); Differential cell slide stain (Fisher cat. no. 122911); Light microscope (Leica); Cytospin 3 (Shandon); 20 ga, 1.25 inch ProtectIV intravenous catheters (Smith Medical); 0.5 mm polymer optical fiber (Edmund Optics # NT02-532).
Polikepahad, S., Barranco, W. T., Porter, P., Anderson, B., Kheradmand, F., Corry, D. B. A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice. J. Vis. Exp. (38), e1720, doi:10.3791/1720 (2010).