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Mitochondrial Isolation for Transmitochondrial Cybrid Generation (Video) | JoVE

Published: March 17, 2023

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Ruth Soler-Agesta, Joaquín Marco-Brualla, Patricio Fernández-Silva², Pilar Mozas³, Alberto Anel, Raquel Moreno Loshuertos²

¹Department of Biochemistry,University of Zaragoza, ²Institute for Biocomputation and Physics of Complex Systems,University of Zaragoza, ³Sequencing and Functional Genomics Unit,University of Zaragoza-IACS

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Mitochondrial Isolation Transmitochondrial Cybrid Generation Centrifugation Homogenization Hypotonic Buffer Mitochondrial Protein Quantification Bradford Assay Nuclear Contamination Assessment Quantitative PCR

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Mitochondrial Isolation for Transmitochondrial Cybrid Generation. J. Vis. Exp. (Pending Publication), e200013, doi:10.3791/200013 (2023).

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