Oral Glucose Tolerance Test (OGTT): Measuring Glucose Metabolism in Mice

Abstract

Source: Ajoy, R. and Chou, S. Y. Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain. J. Vis. Exp. (2018).

This video introduces how to perform an oral glucose tolerance test on a laboratory mouse.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Oral Glucose Tolerance Test (OGTT) Glucose solution preparation: Before conducting the experiment, prepare the glucose solution by dissolving 3.75 g glucose in 15 mL distilled H2O. Set up a timetable to record the readings during the experimental procedure (Table 1). …

Representative Results

Table 1. Timetable for Oral Glucose Tolerance Test (OGTT) recording # Mouse ID Body Glucose Start 0 15 30 60 90 weight µL =10xBW time mins</td…

Materials

Glucometer	Roche	#06870244001	Used to measure the blood glucose level
Glucose chip	Roche	#06454011020	Used to load the blood sample
10 mM glucose			Filter sterilize with a 0.22 µm filter apparatus and store at 4°C.
Animals: C57BL/6	NAR Labs		Wild type mice strain used in the study