Materials
Name | Company | Catalog Number | Comments |
40 μm Cell strainer | Corning | 431750 | |
Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 Dye | Thermo Fisher Scientific | C10337 | |
Cryo freezing container | Nalgene | 5100-0001 | |
CryoStor CS10 | Biolife Solutions | 07930 | cryopreservation medium #1 |
DPBS, no calcium, no magnesium | Thermo Fisher Scientific | 14190144 | |
Genxin | Selcell | YB050050 | cryopreservation medium #2 |
Human embryonic stem cells | provided by Wicell, USA | H9 cell line | |
Matrigel, hESC-Qualified Matrix | Corning | 354277 | basement membrane matrix |
ThawSTAR CFT2 Automated Cell Thawing System | BioLife Solutions | AST-601 | |
Trypan Blue solution 0.4% | Sigma | T8154 | |
TryPLE Select | Thermo Fisher Scientific | 12563029 | cell dissociation reagent |
XVIVO-10 medium | Lonza | BEBP04-743Q | RPE culture medium |
Y-27632 | Selleck | S1049 |
References
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