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Fabrication of Micropipettes for Fluorescence Aspiration Assay to Study Live Cell RBC Mechanosensing

Published: January 12, 2024
doi:
10.3791/201417
, , , , , ,
1School of Biomedical Engineering,The University of Sydney, 2Charles Perkins Centre,The University of Sydney, 3Heart Research Institute, 4The University of Sydney Nano Institute (Sydney Nano),The University of Sydney

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MicropipetteBorosilicate Capillary TubeP-1000 Micropipette PullerHeatVelocityTimePressureMicropipette CutterHeating TemperatureEyepieceAdjustment KnobsBorosilicate Glass BeadHeating PaddleClosed Micropipette TipDiameterFluorescence Aspiration AssayLive Cell RBC Mechanosensing
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Fabrication of Micropipettes for Fluorescence Aspiration Assay to Study Live Cell RBC Mechanosensing. J. Vis. Exp. (Pending Publication), e201417, doi:10.3791/201417 (2024).
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