Hindlimb Skeletal Muscle Extraction: A Procedure to Excise Hindlimb Skeletal Muscles from Murine Model

Abstract

Source: Bonetto, A. et al. The Colon-26 Carcinoma Tumor-bearing Mouse as a Model for the Study of Cancer Cachexia. J. Vis. Exp. (2016)

In this video, we describe the procedure to excise hindlimb skeletal muscles from a mouse model. The excised muscles can be stored at lower temperatures until further downstream analysis. 

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Tissue and Organ Excision

NOTE: For use of tissues in biochemical or molecular biology assays, plan to weigh each organ and tissue and place a fragment immediately into pre-labeled cryotubes. Snap freeze in liquid nitrogen and store at -80 °C.

1. In order to avoid contamination of tissue samples with fur, spray 70% ethanol over the entire body.
2. Place the mouse on a dissection bed in a supine position and extend....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
CD2F1 mice	Harlan	60
Anesthesia apparatus	EZ-Anesthesia	EZ-7000
OCT	Tissue-Tek	4583
Cryostat	Leica	CM1850
Cork disks	Electron Microscopy Sciences	63305
Microtainer	BD	365873
Superfrost plus glass slides	VWR	48311-703
Streptomycin-Penicillin	Cellgro	30-002-CI