Urine Depletion and Bladder Rinsing in Mouse Model: A Catheter-based Procedure to Empty and Wash Murine Urinary Bladder

Abstract

Source: Yu, C. et al. Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation. J. Vis. Exp. (2018)

In this video, we describe a catheter-based procedure to empty and rinse the urinary bladder of a mouse model. The prepped mouse can then be used for further experimental procedures.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

 1. Anesthetizing Animal

1. Use a tabletop anesthesia system to anesthetize the animal with isoflurane. Turn on the O₂ tank and adjust the oxygen flowmeter to 1 L/min.
2. Place an adult female C57BL/6J mouse in the induction chamber. Turn on and adjust the isoflurane vaporizer to 3% for induction. The mouse should be in anesthesia within 2 min. Administer buprenorphine analgesic (0.1 mg/kg) subcutaneously upon removal of the mouse from the ....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
BD 1mL TB Syringe	Fisher	148232E
Exel International Disposable Safelet I.V. Catheters	Fisher Scientific	14-841-21	24G, 2.11 cm length, 0.045 cm outer diameter
K&H Heated Resting Mat for Small Animals, 9 By 12 Inches	Amazon		Cover the heat mat with paper towels
Ophthalmic ointment	Fisher	NC0849514
PBS, pH7.4	Life Technologies	10010049
Pipet tips 200 µL	USA Scientific	1111-0206
Ophthalmic ointment	Fisher	NC0849514
Pipet Tips, Universal Fit; 0.1 to 10 µL	Fisher	02707438(CS)
Buprenorphine	Sigma	B9275-50MG
PIPETMAN NEO P2	Gilson	F144561
PIPETMAN NEO P200N	Gilson	F144565
Therio-gel Veterinary Lubricant	PBS animal health	353-736
V-1 Table top system anesthesia	VetEquip	901806
Isoflurane	Vet one	501017
Tape, Scotch	Fisher	19047257