Intravenous Microinjection of Nephrotoxins into Zebrafish Embryo: A Technique to Deliver Nephrotoxic Agents into the Bloodstream of Zebrafish Embryo

Published: April 30, 2023

Abstract

Source: McKee, Robert A. et al. Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury. J. Vis. Exp. (2016)

This video demonstrates the intravenous microinjection of nephrotoxins into the tail vein of a zebrafish embryo to study their chemical effects on renal function.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Solutions Make a 50x stock solution of E3 embryo media by mixing 73.0 g NaCl, 3.15 g KCl, 9.15 g CaCl2, and 9.95 g MgSO4 in 5 L of distilled water, and store at RT. Make a 50x stock solution of E3 embryo media by mixing 73.0 g NaCl, 3.15 g KCl, 9.15 g CaCl2,…

Disclosures

The authors have nothing to disclose.

Materials

Sodium Chloride  American Bioanalytical  AB01915
Potassium Chloride  American Bioanalytical  AB01652
Magnesium Sulfate  Sigma-Aldrich  M7506
Calcium Chloride  American Bioanalytical  AB00366
N-Phenylthiourea (PTU) Aldrich Chemistry P7629
Ethyl 3-aminobenzoate (Tricaine) Fluka Analytical A5040
Borosilicate glass Sutter Instruments Co. BF100-50-10
Flaming/Brown Micropipette puller Sutter Instruments Co. Mo. P097
UltraPure Agarose Invitrogen 15510-027
Falcon Diposable Petri Dishes, Sterile, Corning:
60 mm x 15 mm VWR 25373-085
100 mm x 15 mm VWR 25373-100
Microinjection tray 150 mm x 15 mm VWR 25373-187
Low Temperature Incubator Fischer Scientific 11 690 516DQ
Micro Dissecting Tweezer Roboz Surgical Instruments Co. RS-5010
Micrometer Ted Pella, Inc. 2280-24

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Cite This Article
Intravenous Microinjection of Nephrotoxins into Zebrafish Embryo: A Technique to Deliver Nephrotoxic Agents into the Bloodstream of Zebrafish Embryo. J. Vis. Exp. (Pending Publication), e20572, doi: (2023).

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