Generation of Porcine Ventricular Slices: A Vibratome-based Technique to Prepare Ultra-thin Slices of Pig Heart Tissue
Published: April 30, 2023
Abstract
Source: Ou, Q. et. al., Slicing and Culturing Pig Hearts under Physiological Conditions. J. Vis. Exp. (2020)
This video demonstrates a vibratome-based procedure to generate viable, ultra-thin sections of pig heart. The prepared tissue slices can be cultured and used as model systems for pharmacological testing.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Pig Heart Tissue Slicing Set up the tissue bath on the vibratome, add ice to the tissue bath cooling jacket, then add Tyrode's solution into the tissue bath. Set up 1 L plastic jar to collect the disposal of melted ice from the tissue bath cooling jacket. For 1 L of the slicing Tyrode's solution, mix 3 g/L 2,3-butanedione monoxi…
Disclosures
The authors have nothing to disclose.
Materials
| Agarose | Bioline USA | BIO-41025 | |
| 2,3-Butanedione monoxime (BDM) | Fisher | AC150375000 | |
| Calcium Chloride (CaCl2) | Fisher | C79-500 | |
| Ceramic Blades for Vibrating Microtome | Campden Instruments | 7550-1-C | |
| D-Glucose | Fisher | D16-1 | |
| Disposable Scalpel #20 | Biologyproducts.com | DS20X | |
| Falcon Cell Strainers, Sterile, Corning | VWR | 21008-952 | |
| Heparin sodium salt | Sigma-Aldrich | H3149-50KU | |
| HEPES | Fisher | BP310-1 | |
| Histoacryl BLUE Tissue glue | Amazon | https://www.amazon.com/HISTOACRYL-FLEXIBLE-1051260P-Aesculap-Adhesive/dp/B074WB5185/ | |
| Iris Spring scissors | Fisher | NC9019530 | |
| Iris Straight Scissors | Fisher | 731210 | |
| Magnesium Chloride (MgCl2) | Fisher | M33-500 | |
| Oxygen regulator | Praxair | ||
| Oxygen tanks | Praxair | ||
| Plastic Pasteur pipettes | Fisher | 13-711-48 | |
| Potassium Chloride (KCl) | Fisher | AC193780010 | |
| Razor rectangle blades | Fisher | 12-640 | |
| Retractable scalpels | Fisher | 22-079-716 | |
| Sodium Bicarbonate (NaHCO3) | Fisher | AC217125000 | |
| Sodium Chloride (NaCl) | Fisher | AC327300010 | |
| Vibrating Microtome | Campden Instruments | 7000 SMZ-2 |
Tags
Cite This Article
Generation of Porcine Ventricular Slices: A Vibratome-based Technique to Prepare Ultra-thin Slices of Pig Heart Tissue. J. Vis. Exp. (Pending Publication), e20837, doi: (2023).