Liquid Marble Microbioreactor System: A 3D Culture Protocol for Encapsulation of Porcine Oocytes
Published: April 30, 2023
Abstract
Source: Gorczyca, G., et al. Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation. J. Vis. Exp. (2020).
In this video, we describe a protocol for the encapsulation of porcine oocytes in hydrophobic microbioreactors.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Encapsulation in super-hydrophobic fluorinated ethylene propylene (FEP) microbioreactors. NOTE: Make sure that all in vitro maturation (IVM) procedures are carried out on a thermostatically controlled table, and cumulus-oocyte complexes (COCs) are maintained at 38 °C throughout their handling. <ol…
Disclosures
The authors have nothing to disclose.
Materials
| Maturation Medium | |||
| hCG (1 VIAL of 10 000 U) | Sigma-Aldrich | CG10 | |
| PMSG | BioVendor | RP1782721000 | |
| DMEM/F12 (500ml) | Sigma-Aldrich | D8062 | Maturation Medium |
| FCS (100 ml) | Thermo Fisher | 16140063 | 10% final concentration for Maturation Medium |
| Liquid Marble | |||
| FEP | Dyneon GmbH 3M AdMD | A-66670 | |
| Specific instruments, tools | |||
| 30 mm Petri dish | TPP | 93040 | |
| 60 mm IVF Petri dish | Falcon | 353653 | |
| Ez-Grid Premium Cell Handling Pipettor | RI Life Sciences | 8-72-288 | |
| Ez-Tip | RI Life Sciences | 8-72-4155/20 | |
| Incubator | Panasonic | MCO-170AIC-PE | Other brands can be used |
| Sterile petri dish (10 cm) | NEST Biotechnology | 704002 |
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Cite This Article
Liquid Marble Microbioreactor System: A 3D Culture Protocol for Encapsulation of Porcine Oocytes. J. Vis. Exp. (Pending Publication), e20844, doi: (2023).