32P-α-GTP |
Perkinelmer |
BLU006X250UC |
|
96 x pin tool |
V&P Scientific |
VP 404 |
96 Bolt Replicator, on 9 mm centers, 4.2 mm Bolt Diameter, 24 mm long |
96-well V-bottom microtiter plate |
Sterilin |
MIC9004 |
Sterilin Microplate V Well 611V96 |
Agar |
OXOID – Thermo Fisher |
LP0011 |
Agar no. 1 |
ASKA collection strain |
NBRP, SHIGEN, JAPAN |
|
Ref: DNA Research, Volume 12, Issue 5, 2005, Pages 291–299. https://doi.org/10.1093/dnares/dsi012 |
Benzonase |
SIGMA |
E1014-25KU |
genetically engineered endonuclease from Serratia marcescens |
Bradford Protein Assay Dye |
Bio-Rad |
5000006 |
Reagent Concentrate |
DMSO |
SIGMA |
D8418 |
≥99.9% |
DNase 1 |
SIGMA |
DN25-1G |
|
gel filtration10x300 column |
GE Healthcare |
28990944 |
contains 20% ethanol as preservative |
Glycerol |
PanReac AppliChem |
122329.1214 |
Glycerol 87% for analysis |
Hypercassette |
Amersham |
RPN 11647 |
20 x 40 cm |
Imidazole |
SIGMA |
56750 |
puriss. p.a., ≥ 99.5% (GC) |
IP Storage Phosphor Screen |
FUJIFILM |
28956474 |
BAS-MS 2040 20x 40 cm |
Isopropyl β-d-1-thiogalactopyranoside (IPTG) |
SIGMA |
I6758 |
Isopropyl β-D-thiogalactoside |
Lysogeny Broth (LB) |
Invitrogen – Thermo Fisher |
12795027 |
Miller's LB Broth Base |
Lysozyme |
SIGMA |
L4949 |
from chicken egg white; BioUltra, lyophilized powder, ≥98% |
MgCl2(Magnesium chloride) |
SIGMA |
208337 |
|
MilliQ water |
|
|
ultrapure water |
multichannel pipette |
Thermo Scientific |
4661110 |
F1 – Clip Tip; 1-10 ul, 8 x channels |
NaCl |
VWR Chemicals |
27810 |
AnalaR NORMAPUR, ACS, Reag. Ph. Eur. |
Ni-NTA Agarose |
Qiagen |
30230 |
|
Nitrocellulose Blotting Membrane |
Amersham Protran |
10600003 |
Premium 0.45 um 300 mm x 4 m |
PBS |
OXOID – Thermo Fisher |
BR0014G |
Phosphate buffered saline (Dulbecco A), Tablets |
PEG3350 (Polyethylene glycol 3350) |
SIGMA |
202444 |
|
phenylmethylsulfonyl fluoride (PMSF) |
SIGMA |
93482 |
Phenylmethanesulfonyl fluoride solution – 0.1 M in ethanol (T) |
Phosphor-imager |
GE Healthcare |
28955809 |
Typhoon FLA-7000 Phosphor-imager |
Pipette Tips, filtered |
Thermo Scientific |
94410040 |
ClipTip 12.5 μl nonsterile |
Poly-Prep Chromatography column |
Bio-Rad |
7311550 |
polypropylene chromatography column |
Protease inhibitor Mini |
Pierce |
A32955 |
Tablets, EDTA-free |
screw cap tube |
Thermo Scientific |
3488 |
Microcentifuge Tubes, 2.0 ml with screw cap, nonsterile |
SLS 96-deep Well plates |
Greiner |
780285 |
MASTERBLOCK, 2 ML, PP, V-Bottom, Natural |
spin column |
Millipore |
UFC500396 |
Amicon Ultra -0.5 ml Centrifugal Filters |
Thermomixer |
Eppendorf |
5382000015 |
Thermomixer C |
TLC plate (PEI-cellulose F TLC plates) |
Merck Millipore |
105579 |
DC PEI-cellulose F (20 x 20 cm) |
Tris |
SIGMA |
BP152 |
Tris Base for Molecular Biology |
Tween 20 |
SIGMA |
P1379 |
viscous non-ionic detergent |
β-mercaptoethanol |
SIGMA |
M3148 |
99% (GC/titration) |