Intravital Microscopy to Analyze Blood Cell-Endothelial Interactions in an Inflammation Mouse Model

Abstract

Source: Herr, N. et al., Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice. J. Vis. Exp. (2015)

In this video, we demonstrate the real-time visualization of blood cell-endothelial interactions in the mesenterial veins of a mouse model injected intraperitoneally with lipopolysaccharide.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Animal Handling, Preparation and Induction of Inflammation

1. Use 4 - 6 week-old male mice with a weight range of 16 - 20 g. Note: If the mice are older or heavier they present excessive fat surrounding the vessel, limiting the microscopic observation.
2. Sterilize all tools and microscope table prior to surgery.
3. Inject 20 mg/kg LPS (lipopolysaccharide) intraperitoneally 4 hr prior to microscopy into the living mouse to induce a bacteri....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Rhodamine 6G	Sigma-Aldrich	989-38-8
Lipopolysaccharides from Escherichia coli 055:B5	Sigma-Aldrich	L2637