蚊子养育的议定书“（冈比亚按蚊）
Summary
该视频演示了在实验室中使用背面按蚊的一般技术。为照顾实验室蚊子的方法证明通过从幼虫到蛹吸血成年人机体的生命周期的所有阶段。
Abstract
这个协议描述在insectary蚊虫饲养。 insectary客房均保持在28 ° C和〜80％湿度，12小时，。日/夜循环。对于此过程，你需要蚊子的笼子里，无菌10％的蔗糖溶液，擦手纸，烧杯，滤纸过滤纸，玻璃进料器，人体血液和血清，水浴，封口膜，蒸馏水，干净的塑料托盘，蚊子的食物（描述下文），蚊帐覆盖的托盘，真空，并收集室，收集成人。
Protocol
蚊子的食物： 食品答：接地的鱼食（Aquaricare）。需要添加一小捏。 食品乙：接地猫粮（普瑞纳）。需要添加一小捏。 食品：猫食品（普瑞纳）。两片需要添加。 血型： 动物或人体血液中可用于后方蚊子。收集了新鲜的血液是用注射器，并把在无菌15毫升猎鹰，每10毫升血液中含有的CPD（抗凝）1毫升管，然后轻轻地混合，在室温下…
Materials
| Material Name | Type | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| Food A | Aquaricare | Grounded fish food. A small pinch needs to be added. | ||
| Food B | Purina | Grounded cat food. A small pinch needs to be added. | ||
| Food C | Purina | Cat food. Two tablets needs to be added | ||
| RBC | prepared red blood cells: 40% hematocrit in human serum | |||
| RPMI | Medium | |||
| CPD | anticoagulant | |||
| serum | O+ human serum | |||
| Mosquito feeder | ||||
| parafilm | ||||
| Anopheles gambiae | mosquitos | |||
| 10% sucrose | mosquito food | |||
| insectary |
References
- Bangs, M. J., Soelarto, T., Barodji, B. P., Wicaksana, D. T., Boewono, Colonization of Anopheles maculatus from Central Java, Indonesia. J Am Mosq Control Assoc. 18, 359-363 (2002).
- Beier, M. S., Beier, J. C., Merdan, A. A., el Sawaf, B. M., Kadder, M. A. Laboratory rearing techniques and adult life table parameters for Anopheles sergentii from Egypt. J Am Mosq Control Assoc. 266, 266-270 (1987).
- Benedict, M. Q., Crampton, J. M., Beard, C. B., Louis, C. . Care and maintenance of anopheline mosquitoes, The molecular biology of disease vectors: A methods manual. , 3-12 (1997).
- Casanges, A. H., Mcgovran, E. R., Chiles, J. V. Rearing of Anopheles quadrimaculatus Say and Aedes aegypti (L.) in the laboratory. Mosq News. 9, 112-117 (1949).
- Merritt, R. W., Dadd, R. H., Walker, E. D. Feeding behavior, natural food and nutritional relation ships of larval mosquitoes. Annual Rev. Entomol. 37, 349-376 (1992).
- Nasirian, H., Ladonni, H. Artificial bloodfeeding of Anopheles stephensi on a membrane apparatus with human whole blood. J Am Mosq Control Assoc. 22, 54-56 (2006).
- Ng’habi, K. R., John, B., Nkwengulila, G., Knols, B. G., Killeen, G. F., Ferguson, H. M. Effect of larval crowding on mating competitiveness of Anopheles gambiae mosquitoes. Malar J. 30 (4), 49 (2005).
- Stobart, R. H. Factors affecting the control of body volume in the larvae of the mosquitoes Aedes aegypti and Aedes detritus. Edw. J. Exp. Boil. 54, 67-82 (1971).
Cite This Article
Das, S., Garver, L., Dimopoulos, G. Protocol for Mosquito Rearing (A. gambiae). J. Vis. Exp. (5), e221, doi:10.3791/221 (2007).