B27 Supplement (50X), serum free |
Thermo Fisher Scientific |
17504-044 |
|
BD FACSAria llu SORP Flow Cytometer |
BD Bioscience |
– |
This has 4 laser system equipped with 405, 488, 594, and 640 nm lasers. |
BD Falcon 70μm Nylon Cell Strainers |
CORNING |
352350 |
For filtering the dissociating cells before FACS. |
BD Falcon Round Bottom Test Tubes With Snap Cap |
CORNING |
352054 |
|
BDNF Human |
ProSpec-Tany TechnoGene, Ltd. |
CYT-207 |
|
Cell Culture microplate, 96 well, PS, F-bottom (Chimney Well) |
Greiner Bio-One International |
655090 |
We tried multiple 96-well dishes and this was the best one for culture and analyses after ICC |
CNTF Human |
ProSpec-Tany TechnoGene, Ltd. |
CYT-272 |
|
4′,6-diamidino-2-phenylinodole (DAPI) |
Thermo Fisher Scientific |
D1306 |
|
Dimethyl sulfoxide |
Sigma-Aldrich |
D2650 |
DMSO |
Dumont #5 Forceps Inox Tip Size .05 x .01 mm Biologie Tips |
Roboz Surgical Instrument |
RS-5015 |
|
Forskolin |
Thermo Fisher Scientific |
BP25205 |
|
GDNF Human |
ProSpec-Tany TechnoGene, Ltd. |
CYT-305 |
|
GlutaMAX supplement |
Thermo Fisher Scientific |
35050-061 |
|
Hanks' Balanced Salt Solution (HBSS) |
Thermo Fisher Scientific |
14175-095 |
|
Hibernate E |
BrainBits |
HE |
|
Hibernate E low fluorescence |
BrainBits |
HELF |
Fluorescence which hinders observation of embryo's GFP expressions should be low. |
Horse serum, heat inactivated, New Zealand origin |
Thermo Fisher Scientific |
26050-070 |
|
IBMX |
Tocris Cookson |
2845 |
Isobutylmethylxanthine |
Laminin |
Thermo Fisher Scientific |
23017-015 |
|
Leibovitz's L15 medium |
Thermo Fisher Scientific |
11415064 |
|
2-Mercaptoethanol |
Sigma-Aldrich |
M6250 |
|
Micro Dissecting Scissors |
Roboz Surgical Instrument |
RS-5913 |
|
Micro Knife 4.75" 1.7 x 27 mm blade |
Roboz Surgical Instrument |
RS-6272 |
|
Moria Mini Perforated Spoon |
Fine Science Tools |
10370-19 |
|
Olympus 1.7ml Microtubes, Clear |
Genesee Scientific |
22-281 |
These are the tubes that we described "1.7 mL microcentrifuge tubes" in the context. |
Papain Dissociation System |
Worthington Biochemical Corp |
LK003150 |
Papain solution and alubumin- ovomucoid inhibitor solution are prepared from this kit. |
Penicillin-streptomycin (10,000 U/ ml) |
Thermo Fisher Scientific |
15140-122 |
|
Phosphate buffered saline (PBS) |
Thermo Fisher Scientific |
10010-023 |
|
Poly D-lysin (PDL) |
MilliporeSigma |
A-003-E |
|
SMZ18 and SMZ1500 zoom stereomicroscopes with DS-Ri1 camera |
Nikon |
– |
Dissection was performed and images of dissected embryos and tissues are captured under these fluorescence microscopes. |
Sylgard 170 Black Silicone Encapsulant – A+B 0.9 Kg kit |
Dow Corning |
1696157 |
We make dissection dishes using this kit. |
TC treated Dishes, 100 x 20 mm |
Genesee Scientific |
25-202 |
We make dissection dishes using this dish. |
Thum Dressing Forceps 4.5" Serrated 2.2 mm Tip Width |
Roboz Surgical Instrument |
RS-8100 |
|
Zeiss LSM 700 series laser scanning confocal microscope and Zen Software |
Carl Zeiss |
– |
Confocal image of the embryo was captured with these equipments |