Insect olfactory systems provide unique opportunities for recording odorant-induced responses in the forms of electroantennograms (EAG) and single sensillum recordings (SSR), which are summed responses from all odorant receptor neurons (ORNs) located on the antenna and from those housed in individual sensilla, respectively.
Abstract
Insect olfactory systems provide unique opportunities for recording odorant-induced responses in the forms of electroantennograms (EAG) and single sensillum recordings (SSR), which are summed responses from all odorant receptor neurons (ORNs) located on the antenna and from those housed in individual sensilla, respectively. These approaches have been exploited for getting a better understanding of insect chemical communication. The identified stimuli can then be used as either attractants or repellents in management strategies for insect pests.
Protocol
1. Insects Navel orangeworm, Amyelois transitella (Walker) (Lepidoptera:Pyralidae) colony in our laboratory originated from moths collected from almond trees in Fresno, CA. Our insects colony is maintained in environmental chambers (Percival Inc, IA ) at 28 ± 2°C, 75 ± 10% relative humidity, and under 16:8h (light:dark) photo regime. Emerging moths are collected daily, sexed, and transferred to plastic boxes (669 mL lunchbox,13 x 13 cm; height, 4.5 cm, Rubbermaid) provid…
Discussion
This novel method developed for restraining a live navel orangeworm moth to measure olfactory signals has proved to be robust and very reliable. We routinely employ this method for isolating and identifying novel attractants from natural host substrates like almonds and pistachios.
Disclosures
The authors have nothing to disclose.
Acknowledgements
Supported by USDA-NIFA/AFRI 2010-65105-20582, NSF 0918177, and Bedoukian Research Incorporation.
Materials
Material Name
Catalogue #/Model
Company
Comment
Microscope
BX51WI model
Olympus, USA
Stereo microscope
MZ75
Leica Microsystems Inc. USA
1.0 mm borosilicate capillary tubing with filament
1B100F-3
WPI Inc, FL
Micropipette puller
P-97
Sutter Instruments, CA
Stimulus controller
CS-55 model
Syntech, Germany
High Impedance pre-amplifiers (Universal Single ended probe)