我々は、この作業中に彼らの親切にサポートし、実り多いアドバイスに理研- BSIで私たちの仲間に感謝します。私は教授重見森、教授G BOR Juh SZ、博士に心から感謝の意を表したいと思います。 Kiyoji松山、総合研究大学院大学、日本の大学院コースの中にマイクロダイアリシス法を勉強する私に助言カタリンAK斯とRP D Dobolyi。最後に、我々は、ビデオ録画での彼らのサポートについては氏クリスSarneckyと博士林明高須に感謝。
Materials
Material Name
Type
Company
Catalogue Number
Comment
Bengalese finches
Animal
Two adult male , Body weight: 14.2 g and 14.8 g
Microdialysis probe
Tool
EICOM
total weight of probe, guide cannula, cap nut and dental adhesive resin: 0.65 to 0.78 g.
Light-cured dental adhesive resin system
Kuraray Medical Inc
+Light cured composite resin (CLEARFIL AP-X).
+Dual-cure dental adhesive system with the self-etching primer and bonding agent (CLEARFIL Liner Bond IIΣ).
Surgical instruments
Stereotaxic frame, surgical microscope, thermal controlled surgical pad and thermal controlled recovery chamber. Plain plugger for handling composite resin. Microspatula or sharp curette to ablate periosteum and muscles.
Injection syringe and needle (30G) for inducing anesthesia.
Small scissors.
Fine forceps for making small craniotomy.
Light-unit
Rolence Enterprise Inc
Ultra-Lite 500E
Microscope
Konan Medical
KOM300
with C-mount adaptor
3- CCD camera
Tool
Hitachi
HV-D30
Photograph 1A shows microdialysis probe (a), a cap nut (b) and a guide cannula (c). In this photo, the guide cannula is surrounded by dental resin which adheres to the skull directly. Photo B shows a bird during drug perfusion. On the top of the head, there is a microdialysis probe that is connected to a fine tefron tube. Scale bars = 1 cm for A and 2 cm for B, respectively.
Figure 1 – Photographs of a microdialysis probe (A) and a bird during drug perfusion (B)
Okumura, T., Okanoya, K., Tani, J. Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments. J. Vis. Exp. (6), e249, doi:10.3791/249 (2007).