常温心脏骤停和心肺复苏术：缺血再灌注损伤的小鼠模型
Summary
一个强大的围手术期和关键护理相关的急性肾损伤模型。使用近复制临床AKI的组织学和功能的变化有可能诱发心脏骤停的全身血流灌注不足。
Abstract
5,6全身低灌注成功繁殖，在啮齿类动物模型急性肾损伤（AKI）是一个共同的，极具杀伤力，危重病的并发症， 死亡率高 1-4，这是最经常全身血流灌注不足引起的。一直困难重重。7-9,9,10聘请局灶性脑缺血模型反复论证的结果没有转化为临床上，和较大的动物模型，全身血流灌注不足无法获得的遗传操纵的工具在鼠标的可能。11,12然而，心脏骤停和心肺复苏的小鼠模型，近年来又出现了可适应模型 AKI的13这种模式可靠地再现生理功能，解剖和组织学在临床AKI的看到的结果，迅速重复，并提供所有手术小鼠模型的显着优点，包括访问的基因操纵技术，成本相对低的大型动物，和易用性。使用这种模式，集团已开发的丰富经验，以评估在AKI的一些器官的具体成果。 14,15
Protocol
按照国家机构的研究和所有动物协议的俄勒冈卫生科学大学的体制的动物护理和使用委员会批准的护理和使用动物的健康指引所述的所有程序进行。 1。手术准备称量鼠标。描述的过程进行C57BL / 6小鼠体重20至25克使用3-4％的异氟醚诱导麻醉诱导，并随后保持1.5-2.5％异氟醚在空气/氧气混合。 润滑眼睛的位置上加热垫的动物仰卧。用胶布固定4四肢。后腿的爪子可…
Discussion
常温下在小鼠心脏骤停和心肺复苏模型提供了一个模式，重复的病理生理及临床最常见的急性肾损伤，全身血流灌注不足引起的形态的多种渠道的评价。假设检验，可访问的遗传操作技术和充分理解和特点的解剖和生理学实验小鼠的一整套资助。
这里描述的，对于有经验的生存率是80％。虽然手术的准备和逮捕后的护理很简单，被捕鼠标的实际复苏是一个具有挑战性的手术技…
Disclosures
The authors have nothing to disclose.
Materials
| Name of the reagent | Company | Catalogue number |
|---|---|---|
| Potassium Chloride | Abbott Medical | 06653-5 |
| Isoflurane | Abbott Medical | 05260-05 (and others) |
| Epinephrine HCl | Multiple | Multiple |
| Digi-Sense temperature controller | Cole-Palmer | EW-89000-00 |
| Angiocath IV Cath | VWR | 381134 |
| Frova angled airway introducer | Cook | G27282 |
| MicroVent Ventilator for Mice | Harvard Apparatus | 733591 |
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Tags
Normothermic Cardiac ArrestCardiopulmonary ResuscitationMouse ModelIschemia-reperfusion InjuryAcute Kidney InjuryCritical IllnessWhole-body HypoperfusionRodent ModelsFocal IschemiaGenetic ManipulationCardiac Arrest And Cardiopulmonary Resuscitation ModelPhysiologic OutcomesFunctional OutcomesAnatomic OutcomesHistologic OutcomesMurine Surgical ModelOrgan-specific Outcomes
Cite This Article
Hutchens, M. P., Traystman, R. J., Fujiyoshi, T., Nakayama, S., Herson, P. S. Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury. J. Vis. Exp. (54), e3116, doi:10.3791/3116 (2011).