ट्राएर मनोवैज्ञानिक तनाव उत्प्रेरण के लिए सामाजिक तनाव टेस्ट प्रोटोकॉल

Summary

इस लेख प्रतिभागियों, जो सक्षम बनाता है शोधकर्ताओं के एक प्रतिभागियों के भीतर या समूहों के बीच मनोवैज्ञानिक तनाव को शारीरिक और neuroendocrine प्रतिक्रियाओं को मापने में मानसिक तनाव उत्प्रेरण के लिए एक प्रोटोकॉल का वर्णन है.

Abstract

This article demonstrates a psychological stress protocol for use in a laboratory setting. Protocols that allow researchers to study the biological pathways of the stress response in health and disease are fundamental to the progress of research in stress and anxiety.¹ Although numerous protocols exist for inducing stress response in the laboratory, many neglect to provide a naturalistic context or to incorporate aspects of social and psychological stress. Of psychological stress protocols, meta-analysis suggests that the Trier Social Stress Test (TSST) is the most useful and appropriate standardized protocol for studies of stress hormone reactivity.² In the original description of the TSST, researchers sought to design and evaluate a procedure capable of inducing a reliable stress response in the majority of healthy volunteers.³ These researchers found elevations in heart rate, blood pressure and several endocrine stress markers in response to the TSST (a psychological stressor) compared to a saline injection (a physical stressor).³ Although the TSST has been modified to meet the needs of various research groups, it generally consists of a waiting period upon arrival, anticipatory speech preparation, speech performance, and verbal arithmetic performance periods, followed by one or more recovery periods. The TSST requires participants to prepare and deliver a speech, and verbally respond to a challenging arithmetic problem in the presence of a socially evaluative audience.³ Social evaluation and uncontrollability have been identified as key components of stress induction by the TSST.⁴ In use for over a decade, the goal of the TSST is to systematically induce a stress response in order to measure differences in reactivity, anxiety and activation of the hypothalamic-pituitary-adrenal (HPA) or sympathetic-adrenal-medullary (SAM) axis during the task.¹ Researchers generally assess changes in self-reported anxiety, physiological measures (e.g. heart rate), and/or neuroendocrine indices (e.g. the stress hormone cortisol) in response to the TSST. Many investigators have adopted salivary sampling for stress markers such as cortisol and alpha-amylase (a marker of autonomic nervous system activation) as an alternative to blood sampling to reduce the confounding stress of blood-collection techniques. In addition to changes experienced by an individual completing the TSST, researchers can compare changes between different treatment groups (e.g. clinical versus healthy control samples) or the effectiveness of stress-reducing interventions.¹

Protocol

1. सेट अप सभी अनुसंधान उपयुक्त संस्थागत समीक्षा बोर्ड या मानव विषयों की समीक्षा डेटा संग्रह करने से पहले समिति द्वारा अनुमोदित किया जाना चाहिए. आदर्श रूप में, दो कमरे TSST आचरण के लिए उपलब्ध होना च?…

Discussion

इस अनुच्छेद में, हम कैसे एक स्वस्थ स्वयंसेवक में Trier सामाजिक तनाव टेस्ट का संचालन करने के लिए प्रदर्शन. TSST एक मानकीकृत प्रयोगशाला सामाजिक stressor है कि मनोवैज्ञानिक, शारीरिक और neuroendocrine उपायों में मजबूत और विश्…

Materials

Name of the equipment/Supply	Company	Catalogue number
BSL Psychophysiology System, Mac OS Including MP36 Data Acquisition Unit and three lead electrocardiogram electrodes	BioPac Systems Inc.	BSLPSY-M
State Trait Anxiety Inventory	Mind Garden, Inc.	STAID-B
Salivary Cortisol Enzyme Immuno Assay Kit	Salimetrics	1-3002
2.0 ml polypropylene vials	Fisher Scientific	05 40B 146
BioRad Microplate Reader, Model 680, with Microplate Manager software	BioRad	Plate Reader: 168 1000 Software: 1706800