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荧光检测方法，为微流体液滴平台

JoVE 신문
생체공학
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JoVE 신문 생체공학

Fluorescence detection methods for microfluidic droplet platforms

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荧光检测方法，为微流体液滴平台

DOI:

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14:16 min

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December 10, 2011

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Xavier Casadevall i Solvas, Xize Niu, Katherine Leeper, Soongwon Cho, Soo-Ik Chang, Joshua B. Edel, Andrew J. deMello

¹Department of Chemistry,Imperial College London , ²Department of Biochemistry, Protein Chip Research Center,Chungbuk National University, ³Department of Chemistry and Applied Biosciences,Institute for Chemical and Bioengineering, ETH Zurich

Chapters

00:05Title
00:27Introduction
02:12Microchip Fabrication
04:07Microfluidic Setup
05:55Optical Setup
08:00Data Acquisition
09:00Cell Recognition
11:13Mapping of Mixing Events Using FLIM
13:04Conclusion

Summary

자동 번역

基于液滴微流控平台的高通量实验的有希望的候选人，因为它们能产生廉价的皮升，自我隔离的船只在千赫率。通过整合，快速，灵敏，高分辨率荧光光谱方法，可以有效地提取这些系统内产生的大量信息，利用和利用。

Tags

Fluorescence Detection Methods Microfluidic Droplet Platforms System Miniaturization Nano-to-femto-liter Volumes Functional Components Integration Large-scale Multiplexing Analytical Throughput Instrumental Footprints Reduction Droplet-based Microfluidic Systems High-throughput Experimentation Water-in-oil Emulsions Capillary Instabilities Microdroplets Generation Isolated Compartments Sample Cross-talk Elimination Dispersion Reduction Minimal Cross-contamination Accurate Timing Of Analytical Processes Contact-free Environment

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