Summary
Halen-suspension test er valideret som en eksperimentel procedure for at vurdere antidepressiv effekt af medicinsk behandling hos mus. Mus er suspenderet af deres haler i seks minutter og flygte-adfærd vurderes. Vi beskriver procedurer, der anvendes på at føre den halen suspension test.
Abstract
Halen-suspensionsprøvning er en mus adfærdsmæssige test nyttige i screening af potentielle antidepressive lægemidler, og vurdering af andre manipulationer, som forventes at påvirke depression adfærd. Mus er suspenderet fra deres haler med tape, i en sådan position, at det ikke kan undslippe eller holde på nærliggende overflader. Under denne test, seks minutters varighed typisk er den resulterende undslippe orienterede adfærd kvantificeret. Halen-suspensionsprøvning er et værdifuldt redskab inden for lægemiddelforskning for high-throughput screening af potentielle antidepressive stoffer. Her beskriver vi de oplysninger, der kræves for gennemførelsen af denne test med ekstra fokus på potentielle problemer, der måtte opstå, og hvordan man kan undgå dem. Vi tilbyder også en løsning på halen klatring adfærd, et fælles problem, der gør denne test ubrugelig i nogle mus stammer, såsom den meget brugte C57BL / 6. Konkret vi forhindre hale klatring adfærd ved at føre musen halen gennem et lille plasticcylinder før suspensionen. Endelig vil vi beskrive, hvordan du manuelt score den adfærd, der er manifesteret i denne test.
Protocol
Discussion
Udvikling af TST ved Steru et al. 9 blev påvirket af den tidligere udviklede tvunget svømme test 10-12. Svarende til det tvungne svømme test, i TST mus er placeret i en uundgåelig, men moderat stressende situation. Mangel på flugt adfærd betragtes som immobilitet. Ligesom tvunget svømme test, er TST en test bedste valideret til vurdering af antidepressive virkning af narkotika, men også bruges til at vurdere virkningerne af miljø-, neurobiologiske og genetiske manipulationer …
Disclosures
The authors have nothing to disclose.
Acknowledgements
Denne undersøgelse er blevet støttet af tilskuddet NiHM R01 MH091816 og R21 MH084043 til TDG.
Materials
- Tape
- Timer
- Video Camera
- White Noise Generator (optional)
- Climbstoppers (optional; depending upon strain used)
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