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Høy oppløsning Live-Imaging of Cell Behavior i utviklingsland Neuroepithelium
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Chapters
- 00:05Title
- 01:33Embryo Electroporation
- 03:04Collagen and Slice Culture Medium Preparation
- 04:22Slice Culture Preparation
- 07:52Imaging Embryo Slices
- 08:58Results: Time-lapse Sequences of Spinal Cord Progenitor Cells
- 10:32Conclusion
Imaging embryonale vev i sanntid er utfordrende over lange perioder. Her presenterer vi en analyse for overvåking cellulære og sub-cellulære endringer i chick ryggmargen for lange perioder med høy romlig og tidsmessig oppløsning. Denne teknikken kan tilpasses for andre regioner i nervesystemet og utvikling av embryo.
Tags
High-resolutionLive ImagingCell BehaviorDeveloping NeuroepitheliumEmbryonic Spinal CordCycling Neural Progenitor CellsNeuronal DifferentiationMolecular MechanismsNeurogenesisReal-time ImagingCell ViabilityCulturePhototoxic EffectsFluorescent ImagingEx Vivo Slice Culture ProtocolWide-field Fluorescence MicroscopyTime-lapse MonitoringSpatial ResolutionTemporal ResolutionCellular BehaviorsSub-cellular BehaviorsGene Activity ReportersNotch Signaling
