Materials
Name | Company | Catalog Number | Comments |
Materials: | |||
Necessary equipment for raising fish and collecting eggs (see the Zebrafish Book22 for details). | |||
Stereomicroscope | Leica | Lecia MZ6 Stereomicroscope | Equipped with laser operating at 488 nm |
Confocal laser scanning microscope | Zeiss | Zeiss LSM 710 NLO | equipped with lasers operating at 488 nm, 561 nm, and 405 nm |
Heating block | |||
Pair of Dumont #5 forceps | |||
Cover-glass-bottomed culture dish | MatTek Corp | Ashland MA USA | |
Microloader Pipette Tips | Eppendorf | ||
Vortex | |||
needles | |||
Reagents: | |||
Egg water medium | |||
60 μg/ml Instant Ocean Sea Salts in ddH2O | |||
E3 medium | |||
5 mM NaCl | |||
0.17 mM KCl | |||
0.33 mM CaCl2 | |||
0.33 mM MgSO4 | |||
0.00001% (w/v) Methylene Blue | |||
4 mg/ml Tricaine stock solution | |||
400 mg Tricaine (3-amino benzoic acidethylester) | Sigma-Aldrich | ||
97.9 ml ddH2O | |||
2.1 ml 1M Tris (pH9) | |||
Adjust at pH 7, aliquot and store -20 °C | |||
1% LMA (Low melting temperature agarose, Lonza) | |||
1g LMA/100 ml E3 medium 1X | |||
Aliquot and store at 4 °C |
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