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1Institute of Pathology, Laboratory of Molecular Tumor Pathology,Charité – Universitätsmedizin Berlin, 2Institute for Chemistry and Biochemistry,Free University Berlin, 3Laboratory for Functional Genomics Charité (LFGC),Charité – Universitätsmedizin Berlin, 4Comprehensive Cancer Center Charité,Charité – Universitätsmedizin Berlin
Chapters
- 00:05Title
- 01:38Preparing Primary Melanoma Single-cells from Tumor Tissue
- 04:33Characterization of Primary Cell Culture and Fibroblast Depletion
- 05:44Magnetic Cell Sorting of CD133+ and CD133- Melanoma Cells
- 09:27Results: Magnetic Cell Sorting of CD133+ and CD133- Melanoma Cells
- 11:38Conclusion
Denne artikkelen beskriver fremstilling av nystekte oppnådd melanom vevet inn primære cellekulturer, og hvordan å fjerne forurensninger av erytrocytter og fibroblaster fra kreftceller. Til slutt beskriver vi hvordan CD133<sup> +</sup> Antatte melanom stamceller er sortert fra CD133<sup> -</sup> Bulk hjelp Magnetic Aktivert Cell Sortering (MACS).
