Name of Reagent/Material |
Company |
Catalog Number |
Comments |
poly-L-lysine slide (ESCO brand) |
Fisher |
12-545-78 |
Sigma brand slides also suitable |
poly-L-lysine hydrobromide |
Sigma |
P1524 |
IMPORTANT: Brand and high molecular weight of polylysine critical |
single frosted edge slides |
Fisher |
48312-002 |
Other brands are suitable |
sodium azide |
Sigma |
S2002-25G |
TOXIC, wear gloves and mask while weighing out powder to make 10% stock. Wear gloves when pipeting stock solution |
coplin staining jar |
VWR |
47751-792 |
Other brands are suitable |
5-slide mailer |
Electron Microscopy Science |
71549-01 |
One of many different brands of small slide holder, suitable for staining |
Paraformaldehyde |
VWR |
MK262159 |
IMPORTANT: Regular formalin solutions (37% formaldehyde) are NOT suitable. Either 1) Dissolve crysatlaline paraformaldehyde in phosphate buffer and store in small aliquots frozen. Or 2) buy electron microscopy grade paraformaldehyde in aliquots. TOXIC -wear gloves; dispose of excess down sink with water. |
Glutaraldehyde solution, 25% in water |
Sigma |
G 5882 |
IMPORTANT: purchase electron microscopy grade glutaraldehyde in ampules; TOXIC -wear gloves; dispose of excess down sink with water. |
Triton X-100 |
VWR |
EM-9410 |
Other brands are suitable |
Bovine Serum Albumin |
Fisher |
ICN820451 |
Other brands are suitable |
Donkey Serum, lyophilized |
Jackson Immunoresearch |
017-000-121 |
Other brands are suitable |
Cy3 Donkey anti-Mouse IgG multi-labeling |
Jackson Immunoresearch |
715-165-150 |
This company is recommended for high quality multi-label (affinity depleted) antibodies. Select appropriate dye and antigen (animal used to raise primary antibody). |
n-propyl gallate |
Fisher |
ICN10274750 |
Other brands are suitable |
DAPI, 4′,6′-diamidino-2-phenylindole dihydrochloride |
Sigma |
D 9542 |
TOXIC. Wear gloves and dispense into small aliquots and feeze to minimize exposure |
Whatman #1 15 cm diameter filters |
Fisher |
09805G |
|
Coverslip #1 1/2 rectangle 24 x 60 mm |
VWR |
48393-252 |
#1 1/2 thickness is optically best |
Microscope slide folder |
VWR |
48429-092 |
Other brands are suitable |