Custom made with a 20 mm diamter hole and a handle to secure o-ring to holder
Upright fluorescence microscope
Olympus America
BX61WI
Image Acquisition Software
Molecular Devices
Metamorph
FITC Dextran 20KD
Sigma Aldrich
0.5 mg/ml (A dextran of different molecular size can be selected, if trial experiments indicate its suitability based on the calculated permeability index values)
Kandasamy, K., Parthasarathi, K. Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation. J. Vis. Exp. (88), e51552, doi:10.3791/51552 (2014).