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测量生活中的斑马鱼胚胎蛋白质稳定性使用荧光衰减光转化后（FDAP）

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Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

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测量生活中的斑马鱼胚胎蛋白质稳定性使用荧光衰减光转化后（FDAP）

DOI:

10.3791/52266-v

•

09:45 min

•

January 28, 2015

•

Katherine W. Rogers, Alexander Bläßle, Alexander F. Schier, Patrick Müller

¹Department of Molecular and Cellular Biology,Harvard University, ²Systems Biology of Development Group,Friedrich Miescher Laboratory of the Max Planck Society

Chapters

00:05Title
01:27Generating a Photoconvertible Fusion Construct and Injecting Dechorionated Zebrafish Embryos
03:22Mounting Zebrafish Embryos for Photoconversion and Confocal Imaging
04:37Photoconverting and Measuring the Decrease of the Photoconverted Signal
06:47Analyzing the Data Using PyFDAP
08:18Results: Half-life of Squint-Dendra2 as Determined by Fluorescence Decay After Photoconversion during Embryogenesis
09:19Conclusion

Summary

자동 번역

在细胞和组织中的蛋白水平通常紧紧蛋白质生产和清除的平衡调节。使用荧光衰减光转化（FDAP）后，蛋白质的间隙动力学可以通过实验在体内测量。

Tags

Protein Stability Fluorescence Decay After Photoconversion FDAP Protein Clearance Kinetics Photoconvertible Fluorescent Protein Zebrafish Embryos Compartmental Analysis Intra- And Extracellular Half-lives Secreted Signaling Proteins In Vivo Protein Dynamics

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