Here, we craft a glass pipette with dual functions: inhibition of deep brain structures by microinjections of drugs and real-time monitoring of their effects through simultaneous electrophysiological recordings.
Supported by grants from CIHR (MOP231122) and NSERC (RGPIN-2014-06503). We would like to thank Geneviève Cyr for her help preparing experiments and supervising laboratory work. MAL received a scholarship from The Natural Sciences and Engineering Research Council of Canada (NSERC).
Materials
Name of the reagent
Company
Catalogue number
Comments (optional)
Injection pump (UltraMicroPump III)
WPI
#UMP3
Injection console (Micro4 Controller)
WPI
#SYS-MICRO4
Hamilton syringe
Hamliton
(80301) 701LT 10 µL SYR
Syringes between 5 and 10 μL used
Gel cyanoacrylate adhesive
Krazy Glue
KG86648R
The gel form is easier to apply on the shaft of the 30G hypodermic needle
Glass pipettes
WPI
#TW100F-4
Thin wall, 1mm OD, 0.75mm ID with filament pipettes used
Lai, J., Legault, M., Thomas, S., Casanova, C. Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain. J. Vis. Exp. (101), e52271, doi:10.3791/52271 (2015).