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多目标显色全安装<em>原位</em>在比较基因表达域杂交<em>果蝇</em>胚胎

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Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

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多目标显色全安装<em>原位</em>在比较基因表达域杂交<em>果蝇</em>胚胎

DOI:

10.3791/53830-v

•

10:17 min

•

January 31, 2016

•

Giselbert Hauptmann, Iris Söll, Robert Krautz, Ulrich Theopold

¹Department of Molecular Biosciences, The Wenner-Gren Institute (MBW),Stockholm University

Chapters

00:05Title
00:55Preparing the Embryos for Hybridization
02:48Hybridization Probes to Prepared Embryos
05:25Detecting the Probes and Staining
07:59Results: Stain Precipitate Color Combinations
09:42Conclusion

Summary

자동 번역

我们描述在完整果蝇胚胎允许同时和特异性检测的三种不同的mRNA分布模式通过对比色沉淀物多靶生色整个安装原位杂交（MC-WISH）过程。

Tags

Multi-target Chromogenic Whole-mount In Situ Hybridization Gene Expression Drosophila Embryos MRNA Localization Multi-color Gene Regulatory Interactions Embryonic Development Organogenesis

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