We described a protocol for prevention of heat stress effects in rats by oral pre-treatment with beneficial bacteria. This protocol can be modified and used for various routes of administration and for analysis of different compounds.
Denne undersøgelse blev designet til at evaluere den beskyttende virkning af Bacillus subtilis stamme mod komplikationer relateret til varmestress. Toogtredive Sprague-Dawley-rotter blev anvendt i denne undersøgelse. Dyr blev oralt behandlet to gange om dagen i to dage med B. subtilis BSB3 stamme eller PBS. Den næste dag efter den sidste behandling blev hver gruppe delt og to forsøgsgrupper (én behandlet med PBS og én behandlet med B. subtilis) blev anbragt ved 45 ° C i 25 min. To kontrolgrupper opholdt sig 25 minutter ved stuetemperatur. Alle rotter blev aflivet og forskellige parametre blev analyseret i alle grupper. Bivirkningerne af varmestress registreres af faldet i villi højde og total slimhinde tykkelse i tarmepitelet; translokation af bakterier fra hulrummet; øget blæredannelse af erythrocytter og elevation af lipopolysaccharider (LPS) i blodet. Den beskyttende effekt af behandlingen vurderes ved prætion af disse bivirkninger. Protokollen blev oprettet til oral behandling af rotter med bakterier til forebyggelse af varme stress komplikationer, men denne protokol kan ændres og anvendes til andre former for indgivelse og til analyse af forskellige forbindelser.
Different stressors affect human and animal health. Temperature is one of the most stressful factors, causing chronic, acute and even lethal illnesses1. Changes in intestinal morphology and a loss of gut barrier integrity after heat stress were documented in many cases2,3. This protective barrier is responsible for defense against translocation of gut bacteria and their toxins, in particular lipopolysaccharides (LPS), from the lumen to the internal circulation4,5. Stability of the gut microbiota significantly influences intestinal barrier function, the immune response of the host, and tolerance to stress conditions6. Thus, modulation of the intestinal microbiota can provide a novel approach for prevention of stress-related adverse effects.
Beneficial bacteria have been used as a promising strategy to modify the gut microbiota for successful management of various clinical conditions, such as diarrhea, inflammatory bowel disease, atopic dermatitis, metabolic disorders7-10. Probiotic effects of beneficial bacteria include production of essential metabolites to support intestinal health, stimulation of the immune system, promotion of lactose tolerance, restoration of epithelial dysfunction. Probiotics were effective in prevention of the stress-related complications in vitro and in animal models11,12.
Researchers have given more attention to Bacillus bacteria as probiotics because these bacteria support intestinal homeostasis13 and have beneficial effects on the host14. Our previous data demonstrated high efficacy of Bacillus probiotics against pathogens in vitro15,16 and in clinical trials17,18. Here, we aim to evaluate the preventive effect of B. subtilis BSB3 strain against complications after heat stress.
Udsættelse for høje temperaturer resulterer i alvorlige sundhedsmæssige betingelser 29. Forebyggelse og tidlig opsporing af komplikationer relateret til varmestress er af afgørende betydning 30. Den præsenterede protokol kan bruges til at evaluere effekten af forskellige tilgange til forebyggelse af varme stress bivirkninger.
Kritiske trin i denne protokol omfatter: varmebehandling procedure (45 ° C, 25 min); brugen af apy…
The authors have nothing to disclose.
This work was supported by the Auburn University fund FOP 101002-139294-2050.
Phosphate buffered saline (PBS) | Sigma-Aldrich, St. Louis, MO | P4417 | |
Ethyl Alcohol | Pharmco products Inc. Brookfield, CT, USA | 64-17-5 | |
Agar | VWR | 97064-334 | |
MacConkey agar plates | VWR | 470180-742 | |
5% blood agar plates | VWR | 89405-024 | |
Brucella blood with Hemin, and Vitamin K, plates |
VWR | 89405-032 | |
Bouin’s Fixative | Electron Microscopy Sciences, Hatfield, PA, USA | 15990 | |
IL-10 Rat ELISA kit | Invitrogen, Camarillo, CA, USA | KRC0101 | |
IL-1beta Rat ELISA Kit | Invitrogen, Camarillo, CA, USA | KRC0011 | |
IL-6 Rat ELISA Kit | Invitrogen, Camarillo, CA, USA | KRC0061 | |
INF-gamma Rat ELISA Kit | Invitrogen, Camarillo, CA, USA | KRC4021 | |
TNF-alpha Rat ELISA Kit | Invitrogen, Camarillo, CA, USA | KRC3011 | |
Rat LPS ELISA Kit | NeoBioLab, MA, USA | RL0275 | |
Environmental Chamber 6020-1 | Caron, Marietta, OH, USA | 6020-1 | |
Centrifuge | Beckman Coulter, Indianapolis, IN, USA | Optima L-90K | |
Ultra Centrifuge | |||
Light microscope optical system | CitoViva Technology Inc., Auburn, AL | ||
Colony counter | Fisher Scientific , Pittsburgh, PA, USA | RE-3325 | |
Freezer | Haier, Brooklyn, NY, USA | HCMO50LA | |
Ultra microplate reader | Bio-Tek Instrument, Winooski, VT, USA | ELx 808 | |
Auto Strip Washer | Bio-Tek Instrument, Winooski, VT, USA | ELx50 | |
Pipettes | Gilson, Pipetman, France | P100, P200, P1000 | |
C24 Incubator Shaker | New Brunswick Scientific, Enfield, CT, USA | Classic C24 | |
Rocking Shaker | Reliable Scientific, Inc., Nesbit, MS, USA | 55 | |
Petri dishes | Fisher Scientific, Pittsburgh, PA, USA | 875713 | 100mmX15mm |
SterilGard III Advance | The Baker Company, Sanford, ME, USA | SG403 | |
Culture Growing Flasks | Corning Incorporated, Corning, NY, USA | 4995 | PYREX 250mL Erlenmeyer flasks |
Optical Spectrometer Genesys 20 | Thermo Scientific, Waltham, MA, USA. | 4001 | |
Sony DXC-33 Video camera | Sony | ||
Richardson test slide | Electron Microscopy Science, Hatfield, PA, USA | 80303 | |
Millipore water purification system | Millipore | Direct-Q | |
Image-Pro Plus software | Media Cybernetics, MD, USA | ||
Triple Beam Balance | OHAUS Corporation, Parsippany, NJ, USA | ||
Tissue Homogenizer, Dounce | VWR | 71000-518 | |