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体外在拟南芥中受精卵分化和胚模式的活体细胞成像的胚珠培养

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In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

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体外在拟南芥中受精卵分化和胚模式的活体细胞成像的胚珠培养

DOI:

10.3791/55975-v

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05:41 min

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September 11, 2017

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Daisuke Kurihara*^1,2, Yusuke Kimata*¹, Tetsuya Higashiyama^2,3, Minako Ueda³

¹Division of Biological Science, Graduate School of Science,Nagoya University, ²Higashiyama Live-Holonics Project, JST-ERATO,Nagoya University, ³Institute of Transformative Bio-Molecules (ITbM),Nagoya University

Chapters

00:05Title
00:45Poly(Dimethylsoloxane) Micropillar Array Divide Preparation
02:03Ovule Extraction and Time-lapse Imaging
04:14Results: Representative Live-imaging of Zygote Polarization and Embryo Patterning
05:03Conclusion

Summary

자동 번역

这份手稿描述了一个体外胚珠培养方法, 使受精卵和胚胎的活细胞成像的拟南芥。该方法用于在受精卵分化过程中对细胞内动力学进行可视化, 并对发育中的胚胎的胞命运规范进行研究。

Tags

In Vitro Ovule Cultivation Live-cell Imaging Zygote Polarization Embryo Patterning Arabidopsis Thaliana PDMS Micropillar Array N5T Medium Silique Dissection Ovule Transfer Cover Glass

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