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Vollständig-hängen Clearing und Färbung des Arabidopsis Blume Organe und Siliques
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JoVE 신문 발생학
Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
DOI:

09:17 min

April 12, 2018

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Chapters

  • 00:04Title
  • 00:56Flower and Silique Fixation and Dissection Under the Stereomicroscope
  • 02:05Chloral Hydrate-based Clearing and Combined Clearing-staining
  • 03:28Combined Alexander Staining and Herr’s 4½ Clearing of Anthers
  • 04:29Removing the Exine from Pollen Grains
  • 05:49Sodium Dodecyl Sulfate (SDS) Clearing
  • 07:04Results: Arabidopsis Flowers and Floral Organs are Dissected, Cleared, and Stained for Whole Mount Observations of Reproductive Structures
  • 08:41Conclusion

Summary

자동 번역

In diesem Protokoll wir beschreiben Techniken für die richtige Zerlegung von Arabidopsis Blumen und Siliques, einige grundlegende Clearing-Techniken und Färbung Verfahren für ganz-Mount Beobachtungen der reproduktiven Strukturen ausgewählt.

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