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体外激酶法鉴定细胞周期蛋白依赖性激酶1特异磷酸化部位
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JoVE 신문 생화학
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
DOI:

12:26 min

May 03, 2018

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Chapters

  • 00:04Title
  • 01:25Prediction of Cdk1-specific Phosphorylation Sites and Expression of CENP-F in Escherichia coli
  • 04:11Recombitant Protein Purification by Glutathione-affinity Chromatography and Gel Filtration Followed by In Vitro Kinase Assay with Cdk1/Cyclin B
  • 09:50Results: Use of In Vitro Kinase Assay to Study Cdk1-specific Phosphorylation Sites in CENP-F
  • 11:13Conclusion

Summary

자동 번역

细胞周期蛋白依赖性激酶 1 (Cdk1) 在 G2 阶段被激活并且调控许多细胞通路。在这里, 我们提出了一个协议的体外激酶检测与 Cdk1, 这使得识别 Cdk1-specific 磷酸化的网站, 以建立细胞目标的这个重要的激酶。

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