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牺牲纤维膜细胞培养生产细胞外基质纤维

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Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture

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牺牲纤维膜细胞培养生产细胞外基质纤维

DOI:

10.3791/58791-v

•

06:01 min

•

February 02, 2019

•

Kevin Roberts, John Taehwan Kim, Shelby White, Jamie Hestekin, Jeffrey C. Wolchok

¹Cell & Molecular Biology Program,University of Arkansas, ²Department of Biomedical Engineering,University of Arkansas, ³Ralph E. Martin Department of Biomedical Engineering,University of Arkansas

Chapters

00:04Title
00:34Hollow Fiber Membrane (HFM) Cell Seeding and Extracellular Matrix (ECM) Extraction
03:06Extracellular Matrix Decellularization
04:25Results: Representative ECM Implant Assembly
05:21Conclusion

Summary

자동 번역

该方案的目标是生产针对伤口修复的整体细胞外基质纤维, 作为再生支架植入物的一部分, 适合临床前评估。这些纤维是通过在中空纤维膜上培养成纤维细胞而产生的, 并通过膜的溶解提取。

Tags

Extracellular Matrix Sacrificial Hollow Fiber Membrane Cell Culture Fibroblasts Wound Repair Biomaterial Textile Fabrication N-Methyl-2-pyrrolidone Silicone Rubber Mold

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