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1Department of Medicine, Division of Hematology/Oncology, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Rutgers School of Graduate Studies at New Jersey Medical School,Rutgers Biomedical and Health Sciences, 3Department of Surgery, Division of Plastic Surgery, New Jersey Medical School,Rutgers Biomedical and Health Sciences
Chapters
- 00:04Title
- 01:10Isolation of ASCs from Adipose Tissue
- 02:37Culture of ASCs
- 03:29Results: An Explant-based Enzyme-free Method of ASCs Isolation
- 04:02Conclusion
Denne protokol giver en enzym-fri metode til isolering af mesenchymale stamceller fra mave og lipoaspirat prøver ved hjælp af en forklarings metode. Fraværet af barske enzymer eller Centrifugerings trin giver klinisk relevante stamceller, der kan anvendes til undersøgelser in vitro eller overføres tilbage til klinikken.
