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Live Cell Imaging af Microtubule Cytoskeleton og mikromekanisk manipulation af Arabidopsis Skyd Apical Meristem
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Developmental Biology
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JoVE Journal Developmental Biology
Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

Live Cell Imaging af Microtubule Cytoskeleton og mikromekanisk manipulation af Arabidopsis Skyd Apical Meristem

DOI:
10.3791/60936-v

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07:52 min

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May 23, 2020

•

,
1Max Planck Institute of Molecular Plant Physiology

Chapters

  • 00:04Introduction
  • 00:34Plant Growth
  • 01:18Shoot Apical Meristem (SAM) Dissection
  • 02:14Cultured SAM Transfer and Growth
  • 02:57SAM Imaging
  • 04:57Micromechanical SAM Perturbation and Data Analysis
  • 06:23Results: Representative Cortical Microtubule Anisotropy Quantification and Mechanical Ablation in Arabidopsis SAM
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en protokol for levende celle billeddannelse af kortikale mikrotubule cytoskeleton på skyde apical meristem og overvåge dens reaktion på ændringer i fysiske kræfter.

Tags

Microtubule CytoskeletonLive Cell ImagingArabidopsisShoot Apical MeristemMechanical ManipulationPlant GrowthPlant DevelopmentDissectionMicroscopyPlant Genetics

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