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Billeddirigeret bioreaktor til generering af biomanirigeret luftvejsvæv
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JoVE Journal Bioengineering
Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue

Billeddirigeret bioreaktor til generering af biomanirigeret luftvejsvæv

DOI:
10.3791/63544-v

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11:01 min

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April 06, 2022

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, , , , , ,
1Department of Biomedical Engineering,Stevens Institute of Technology, 2Department of Biomedical Engineering,Columbia University, 3Department of Cell Biology,State University of New York Downstate Medical Center, 4Department of Cardiothoracic Surgery,Stanford University

Chapters

  • 00:05Introduction
  • 01:14Construction of Gradient Index (GRIN) Lens-Based Imaging Device
  • 02:25Imaging-Guided Removal of the Trachea Epithelium
  • 05:51Homogeneous Distribution of Exogenous Cells onto the Denuded Tracheal Lumen
  • 08:23Results: Imaging-Guided Bioreactors for the Generation of Functional In Vitro Airway Tissues
  • 10:18Conclusion

Summary

Automatic Translation

Automatic Translation

Protokollen beskriver en billeddannelsesaktiveret bioreaktor, der muliggør selektiv fjernelse af det endogene epitel fra rotteluftrøret og homogen fordeling af eksogene celler på lumenoverfladen efterfulgt af langvarig in vitro-kultur af cellevævskonstruktionen.

Tags

Imaging-guided BioreactorIn Vitro CultivationMicroscopic VisualizationTracheal LumenDe-epithelizationIn Situ ImagingDisease ModelingDrug ScreeningCMOS CameraOptical Cage SystemObjective LensCarboxyfluorescein Succinimidyl Ester (CFSE)PBS WashTrachea CannulaSyringe Pump

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