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Chapters
- 00:17Introduction
- 01:10Dissection of the Bulge from Adult Mouse Whisker Follicles
- 04:34Culture of Bulge Explants
- 07:18Conclusion
Hier zeigen wir unsere Methode zur Maus epidermalen Neuralleiste Stammzellen (EPI-NCSC) zu isolieren. Technik beinhaltet Mikro-Sezieren Whisker Follikel, die Isolierung des Bulge und Silber reflektiert sie in Gewebekultur. EPI-NCSC beginnen mit Ausbuchtung Explantate auf das Substrat innerhalb von 3 auswandern - 4 Tage.
Tags
Mouse Epidermal Neural Crest Stem CellEPI-NCSCEmbryonic Neural CrestHair FolliclesMultipotent Stem CellsDifferentiated Cell TypesNeuronsNerve Supporting CellsSmooth Muscle CellsBone/cartilage CellsMelanocytesHairy SkinPure Population Of Stem CellsProtocolWhisker FolliclesWhisker PadCollagen CapsuleCulture PlateEmigration
