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Chapters
- 00:00Title
- 00:53Introduction
- 01:50Preparing The Peptides
- 03:32Solubilizing and Photo-Cross-Linking the HFIP-Treated Peptide
- 06:22Representative Result
- 07:54Conclusion
Photo-induzierte Quervernetzung von unmodifizierten Proteine (picup) ermöglicht die Charakterisierung des Oligomers Größenverteilung in metastabilen Protein-Mischungen. Wir zeigen, Anwendung picup zu drei Vertreter amyloidogene Peptide der 40 - und 42-Rückstand bildet der Amyloid-β-Protein und Calcitonin, und eine Kontrollgruppe Peptid Wachstumshormon-Releasing-Faktor.
Tags
Photo-Induced Cross-Linking Of Unmodified ProteinsPICUPAmyloidogenic PeptidesProtein Misfolding DiseasesAlzheimer’s DiseaseParkinson’s DiseaseHuntington’s DiseaseHereditary Amyotrophic Lateral SclerosisType 2 DiabetesOligomer Size DistributionClassical MethodsElectrophoresisChromatographyFluorescenceDynamic Light ScatteringMetastable MixturesCovalent Cross-linkingSodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)Size-exclusion Chromatography (SEC)VisualizationQuantitative AnalysisAssembly MonitoringSequence ModificationsOligomerization Mechanism
