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Chapters
- 00:00Title
- 00:53Introduction
- 01:50Preparing The Peptides
- 03:32Solubilizing and Photo-Cross-Linking the HFIP-Treated Peptide
- 06:22Representative Result
- 07:54Conclusion
Foto-inducido de entrecruzamiento de las proteínas sin modificar (picup) permite la caracterización de la distribución del tamaño de oligómero de mezclas de proteínas metaestable. Se demuestra la aplicación de picup a tres péptidos representante amiloidogénica los 40 - y 42-residuo forma de amiloide β-proteína, y la calcitonina, y un péptido de control de la hormona del crecimiento factor de liberación.
Tags
Photo-Induced Cross-Linking Of Unmodified ProteinsPICUPAmyloidogenic PeptidesProtein Misfolding DiseasesAlzheimer’s DiseaseParkinson’s DiseaseHuntington’s DiseaseHereditary Amyotrophic Lateral SclerosisType 2 DiabetesOligomer Size DistributionClassical MethodsElectrophoresisChromatographyFluorescenceDynamic Light ScatteringMetastable MixturesCovalent Cross-linkingSodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)Size-exclusion Chromatography (SEC)VisualizationQuantitative AnalysisAssembly MonitoringSequence ModificationsOligomerization Mechanism
