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Chapters
- 00:00Title
- 00:58Introduction
- 01:27Disruption of the Influenza A Virion
- 02:53Glycerol Gradient Sediment Velocity Centrifugation
- 04:18Analysis of the Glycerol Gradient Fractions by SDS Polyacrylamide Gel Electrophoresis
- 05:40Concentration of the vRNP Fractions
- 06:39Negative Staining of vRNPs
- 08:25Representative Results/ Outcome
- 09:05Conclusion
Le génome du virus de la grippe A se compose de huit complexes séparés d'ARN et de protéines, appelées complexes ribonucléoprotéiques virale (vRNPs). Ce document décrit la purification sur gradient de glycérol et de visualisation microscopie électronique en transmission de la grippe A vRNPs.
Tags
PurificationVisualizationInfluenza AViral Ribonucleoprotein ComplexesVRNPsViral EnvelopeNuclear ImportReplicationIsolated VRNPsProcedureDisruptionDetergentsGlycerol GradientVelocity SedimentationSDS-PAGECoomassie Blue StainingPeak FractionsConcentrationCentrifugationIntegrity VerificationTransmission Electron Microscopy
